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6-amino-3-methyl-2-(methylthio)pyrimidin-4(3H)-one; CAS No.: 54030-56-7; ChemShuttle s 6-amino-3-methyl-2-(methylthio)pyrimidin-4(3H)-one serves as a conformationally restricted thymine analog. The methyl group at N3 prevents glycosidic bond rotation, while the thioether enhances lipophilicity. Our DNA incorporation studies use radiolabeled compounds to measure replication errors.