5'-O-DMT-N4-Ac-dC
$400.00
CAS No.: 100898-63-3
Catalog No.: 198460
Purity: 95%
MF: C32H33N3O7
MW: 571.63
Storage: 2-8 degree Celsius
SMILES: COC1=CC=C(C=C1)C(OC[C@@H]1[C@H](C[C@@H](O1)N1C(N=C(C=C1)NC(C)=O)=O)O)(C1=CC=CC=C1)C1=CC=C(C=C1)OC
Catalog No.: 198460
Purity: 95%
MF: C32H33N3O7
MW: 571.63
Storage: 2-8 degree Celsius
SMILES: COC1=CC=C(C=C1)C(OC[C@@H]1[C@H](C[C@@H](O1)N1C(N=C(C=C1)NC(C)=O)=O)O)(C1=CC=CC=C1)C1=CC=C(C=C1)OC
CAS No.: 100898-63-3; 5'-O-DMT-N4-Ac-dC. PROPERTIES: 5'-O-DMT-N4-Ac-dC is a protected deoxycytidine derivative with molecular formula C31H30N3O9P and molecular weight 645.56 g/mol. It typically appears as a white powder with limited aqueous solubility, requiring chloroform or methanol for dissolution. The compound is sensitive to light and moisture, necessitating storage at -20 C in a desiccated, amber glass container. Safety precautions include handling in a chemical fume hood and using protective equipment to prevent skin and eye contact. APPLICATIONS: In oligonucleotide synthesis, 5'-O-DMT-N4-Ac-dC serves as a building block for automated DNA synthesis using phosphoramidite chemistry. The dimethoxytrityl (DMT) group protects the 5'-hydroxyl group during coupling reactions and is removed under acidic conditions, while the N4-acetyl group protects the amino function and is typically cleaved during final deprotection. This protection strategy allows sequential coupling of nucleotides to form custom DNA sequences with high fidelity. In molecular biology, it is used to synthesize primers and probes for PCR, sequencing, and hybridization assays. Additionally, the compound enables incorporation of modified cytidine residues into oligonucleotides for studying DNA-protein interactions and for developing therapeutics with enhanced nuclease resistance. The DMT protection facilitates monitoring of coupling efficiency during synthesis through UV absorbance measurement. In chemical biology, the protected dC is utilized in developing nucleic acid analogs that modulate gene expression through antisense mechanisms or RNA interference. (Oligonucleotide synthesis manuals and molecular biology research literature)
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