4-Methylumbelliferyl b-D-glucosaminide
$1,200.00
CAS No.: 58989-36-9
Catalog No.: 197761
Purity: 95%
MF: C16H19NO7
MW: 337.328
Storage: 2-8 degree Celsius
SMILES: N[C@H]1[C@@H](O[C@@H]([C@H]([C@@H]1O)O)CO)OC1=CC=C2C(=CC(OC2=C1)=O)C
Catalog No.: 197761
Purity: 95%
MF: C16H19NO7
MW: 337.328
Storage: 2-8 degree Celsius
SMILES: N[C@H]1[C@@H](O[C@@H]([C@H]([C@@H]1O)O)CO)OC1=CC=C2C(=CC(OC2=C1)=O)C
4-Methylumbelliferyl b-D-glucosaminide; 7-(((2S,3R,4R,5S,6R)-3-amino-4,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-4-methyl-2H-chromen-2-one; CAS No.: 58989-36-9; 4-Methylumbelliferyl b-D-glucosaminide. PROPERTIES: 4-Methylumbelliferyl b-D-glucosaminide is a fluorescent substrate with molecular formula C15H15NO7 and molecular weight 345.3 g/mol. This compound typically appears as a white to off-white powder with a melting point of approximately 158-162 C. It exhibits moderate water solubility and is sensitive to light and moisture. Proper storage requires a tightly sealed container in a cool, dark place at 2-8 C, preferably under inert atmosphere to prevent degradation. Safety precautions include wearing protective gloves, eye protection, and laboratory coat when handling, as it may cause eye irritation upon contact. APPLICATIONS: In biochemical research, 4-Methylumbelliferyl b-D-glucosaminide serves as a valuable substrate for measuring -glucosaminidase enzyme activity. The compound is hydrolyzed by the enzyme to release 4-methylumbelliferone, which fluoresces intensely under UV light (excitation ~360 nm, emission ~450 nm), allowing quantitative analysis of enzyme kinetics. This application is widely used in studies of lysosomal enzyme function and in screening for enzyme inhibitors or activators. Additionally, it finds utility in cellular biology for monitoring lysosomal activity and in pharmacology for evaluating drug effects on glycosaminidase functions. The compound's fluorescence properties make it particularly useful in high-throughput screening assays for potential therapeutic agents targeting glycosaminoglycan metabolism disorders. (Biochemistry journals and enzyme assay protocols)
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