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X-Gluc cyclohexanamine; cyclohexanamine(2S,3S,4S,5R,6S)-6-((5-bromo-4-chloro-1H-indol-3-yl)oxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylate; CAS No.: 114162-64-0;X-Gluc cyclohexanamine. PROPERTIES: X-Gluc cyclohexanamine is a white or off-white crystalline solid with a molecular formula of C23H24N2O10 and a molecular weight of 492.44 g/mol. It has limited aqueous solubility but dissolves in dimethylformamide. Storage should be at 4 C in a sealed container protected from moisture. When handling, chemical-resistant gloves and eye protection are recommended due to potential skin and eye irritation. APPLICATIONS: X-Gluc is a chromogenic substrate used in beta-glucuronidase (GUS) assays. In molecular biology, it enables visualization of GUS reporter gene expression in transgenic organisms through blue color development upon enzymatic cleavage (source: Analytical Biochemistry). The cyclohexanamine form enhances its stability in storage and handling. Additionally, X-Gluc serves in microbiology for detecting bacterial GUS activity, aiding in strain identification and functional studies (source: Journal of Microbiological Methods).