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Thymidine; 1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione; CAS No.: 50-89-5;Thymidine. PROPERTIES: Thymidine is a white or almost white crystalline powder with a molecular formula of C10H14N2O5 and a molecular weight of 242.23 g/mol. It is highly soluble in water and has a slightly acidic pH. Storage should be at room temperature in a tightly closed container. Safety precautions include avoiding ingestion and eye contact, with standard laboratory handling procedures. APPLICATIONS: Thymidine is a natural nucleoside component of DNA. In molecular biology, it serves as a building block for DNA synthesis and is used in cell culture media to support cell proliferation (source: Nucleic Acids Research). Radiolabeled thymidine is employed in studying DNA replication kinetics and cell proliferation rates in various tissues. Additionally, it functions as a substrate for studying DNA polymerase activity and fidelity in enzymatic assays (source: Biochemistry).