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EGTA tetrasodium; sodium 3,12-bis(carboxylatomethyl)-6,9-dioxa-3,12-diazatetradecanedioate; CAS No.: 13368-13-3; EGTA tetrasodium. PROPERTIES: EGTA tetrasodium is a calcium chelating agent appearing as a white to off-white crystalline powder. This EGTA tetrasodium compound has a molecular weight of 462.48 g/mol with a pH range of 7-9 in solution. It is highly soluble in water but insoluble in most organic solvents. The substance is hygroscopic and should be stored in a tightly sealed container at room temperature. It is advisable to avoid exposure to high temperatures and moisture. In terms of safety, EGTA tetrasodium may cause eye irritation and skin irritation, so protective measures are recommended during handling. APPLICATIONS: EGTA tetrasodium is primarily used in biological research to control intracellular calcium levels. It serves as a calcium ion buffer in cell culture media and electrophysiological experiments. In molecular biology, EGTA tetrasodium is utilized to inhibit calcium-dependent enzymes such as calmodulin and protein kinase C. It also finds application in diagnostic assays for measuring calcium-regulated processes. In pharmacological studies, it helps elucidate calcium signaling pathways and their role in disease mechanisms. These applications are frequently cited in cell biology literature and calcium signaling research publications.